8JCA
Cyrstal structure of SKIP RUN domain in complex with GTP-bound Arl8b(Q75L)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-20 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 178.687, 53.836, 40.512 |
Unit cell angles | 90.00, 98.13, 90.00 |
Refinement procedure
Resolution | 32.050 - 1.650 |
R-factor | 0.1761 |
Rwork | 0.175 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.034 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHENIX ((1.17.1_3660: ???)) |
Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.504 | 1.680 |
High resolution limit [Å] | 1.647 | 1.650 |
Rmerge | 0.775 | |
Number of reflections | 41074 | 41074 |
<I/σ(I)> | 0.073 | |
Completeness [%] | 89.1 | |
Redundancy | 2.7 | |
CC(1/2) | 0.990 | 0.400 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 800mM Potassiam phosphate dibasic 1.2 M Sodium phosphate monobasic 0.1M CAPS/Sodium hydroxide 0.2M Lithium sulfate pH10.5 |