8JBC
Crystal Structure of the Csm6 K137A mutant from Thermus thermophilus HB8 in its apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL10U2 |
Synchrotron site | SSRF |
Beamline | BL10U2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2022-08-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 94.890, 205.570, 59.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.640 - 2.410 |
R-factor | 0.2242 |
Rwork | 0.222 |
R-free | 0.26930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.112 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 (1.20.1_4487) |
Phasing software | MOLREP |
Refinement software | REFMAC (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.520 | 2.470 |
High resolution limit [Å] | 2.410 | 2.410 |
Number of reflections | 45579 | 4483 |
<I/σ(I)> | 15 | |
Completeness [%] | 99.9 | 99.93 |
Redundancy | 12.9 | |
CC(1/2) | 0.999 | 0.676 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 0.01 M Magnesium chloride hexahydrate, 0.1M HEPES sodium pH 7.0, 15% w/v Polyethylene glycol 3350 |