Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 122.520, 122.520, 119.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.510 - 2.040 |
| R-factor | 0.1674 |
| Rwork | 0.167 |
| R-free | 0.17210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.638 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.510 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.059 | 1.231 |
| Rmeas | 0.062 | 1.286 |
| Rpim | 0.018 | 0.370 |
| Number of reflections | 42695 | 4254 |
| <I/σ(I)> | 22.19 | 1.83 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12 | 12.1 |
| CC(1/2) | 1.000 | 0.803 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M sodium acetate, pH4.5-pH4.9, 24-30% w/v PEG 4000, 15% v/v glycerol |
