8J8D

Crystal structure of SRCR domain 11 of DMBT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-24
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00
Spacegroup name	P 21 21 2
Unit cell lengths	81.802, 31.705, 35.219
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.900 - 1.510
R-factor	0.13999
Rwork	0.138
R-free	0.17225
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6s5a
RMSD bond length	0.011
RMSD bond angle	1.714
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.900	4.490
High resolution limit [Å]	1.510	1.510
Number of reflections	22788	3604
<I/σ(I)>	27.02
Completeness [%]	98.7
Redundancy	2.94
CC(1/2)	0.999	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293.15	0.2 M Ammonium iodide, 20% PEG3350