8J71

Selenomethionine mutant (L601Sem/L654Sem) of SAND domain of protein SP140 with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	110
Detector technology	PIXEL
Collection date	2020-10-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	41.131, 59.748, 124.956
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.070 - 1.850
R-factor	0.1956
Rwork	0.194
R-free	0.22350
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.015
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.880
High resolution limit [Å]	1.850	5.020	1.850
Rmerge	0.127	0.095	0.673
Rmeas	0.136	0.101	0.712
Rpim	0.046	0.034	0.230
Number of reflections	26673	1508	1283
<I/σ(I)>	5.1
Completeness [%]	98.3	99.6	96.4
Redundancy	8.7	8.7	9
CC(1/2)	1.000	0.994	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.12 M DL-Malic acid pH 7.0, 14% w/v PEG 3350 0.025% v/v Dichloromethane