8J71
Selenomethionine mutant (L601Sem/L654Sem) of SAND domain of protein SP140 with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2020-10-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.131, 59.748, 124.956 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.070 - 1.850 |
R-factor | 0.1956 |
Rwork | 0.194 |
R-free | 0.22350 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.015 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 5.020 | 1.850 |
Rmerge | 0.127 | 0.095 | 0.673 |
Rmeas | 0.136 | 0.101 | 0.712 |
Rpim | 0.046 | 0.034 | 0.230 |
Number of reflections | 26673 | 1508 | 1283 |
<I/σ(I)> | 5.1 | ||
Completeness [%] | 98.3 | 99.6 | 96.4 |
Redundancy | 8.7 | 8.7 | 9 |
CC(1/2) | 1.000 | 0.994 | 0.910 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.12 M DL-Malic acid pH 7.0, 14% w/v PEG 3350 0.025% v/v Dichloromethane |