8J6H
Structure and allosteric regulation of the inosine 5'-monophosphate-specific phosphatase ISN1 from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97926 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 147.391, 165.061, 82.672 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.089 - 2.441 |
R-factor | 0.236825102162 |
Rwork | 0.236 |
R-free | 0.27881 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.731 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.440 | 2.450 |
Rmerge | 0.110 | |
Number of reflections | 74898 | 7175 |
<I/σ(I)> | 9.34 | |
Completeness [%] | 99.5 | 96.5 |
Redundancy | 6.4 | 5.5 |
CC(1/2) | 0.990 | 0.480 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295.15 | 0.26M ammonium dihydrogen phosphate, 35% glycerol |