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The crystal structure of bat coronavirus RsYN04 RBD bound to the antibody S43

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 43 21 2
Unit cell lengths	68.373, 68.373, 240.863
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.550 - 3.000
R-factor	0.2275
Rwork	0.227
R-free	0.23060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.766
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.000	3.000
High resolution limit [Å]	3.000	3.000
Rmerge	0.123	1.819
Number of reflections	21699	2255
<I/σ(I)>	20.3
Completeness [%]	99.8
Redundancy	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Potassium nitrate, 20% w/v Polyethylene glycol 3,350