8J5I
MALT1 death domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2019-12-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 35.232, 83.229, 85.403 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.350 - 2.100 |
R-factor | 0.2191 |
Rwork | 0.218 |
R-free | 0.24070 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.867 |
Data reduction software | HKL-2000 (HKL-2000) |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.046 | 0.487 |
Rmeas | 0.054 | 0.568 |
Rpim | 0.028 | 0.289 |
Number of reflections | 7761 | 728 |
<I/σ(I)> | 14.8 | 2.98 |
Completeness [%] | 99.2 | |
Redundancy | 3.6 | |
CC(1/2) | 0.990 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | PEG 3350, Tacsimate pH 6 |