8J59
The structure of a novel thermophilic-like old yellow enzyme from Aspergillus flavus-AfOYE1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.473, 171.422, 50.478 |
| Unit cell angles | 90.00, 117.60, 90.00 |
Refinement procedure
| Resolution | 42.860 - 1.550 |
| R-factor | 0.1451 |
| Rwork | 0.144 |
| R-free | 0.16740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.924 |
| Data scaling software | XDS (version 1.12 (25-MAR-2022)) |
| Refinement software | PHENIX (1.20.1-4487_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.860 | 1.605 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.050 | 0.170 |
| Rmeas | 0.050 | 0.190 |
| Rpim | 0.020 | 0.080 |
| Number of reflections | 102393 | 9786 |
| <I/σ(I)> | 25.71 | 7.66 |
| Completeness [%] | 99.3 | 95.09 |
| Redundancy | 6.5 | 5.5 |
| CC(1/2) | 0.999 | 0.980 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1 M Bis-Tris propane, pH 6.5, 15% (W/v) PEG3350, 0.2 M sodium nitrate |
