8J30

Crystal structure of ApNGT with Q469A and M218A mutations in complex with UDP-GLC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	79.409, 95.011, 175.974
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.180 - 2.890
R-factor	0.2134
Rwork	0.211
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.273
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.930
High resolution limit [Å]	2.880	2.880
Number of reflections	30111	28618
<I/σ(I)>	13.9
Completeness [%]	98.8
Redundancy	6.2
CC(1/2)	0.985	0.721

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M TBis-Tris-HCl (pH 6.5), 2%(v/v) Tacsimate(pH 6.0), 20%(v/v) PEG 3350