8J2W
Saccharothrix syringae photocobilins protein, dark state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-14 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.111, 97.539, 72.662 |
| Unit cell angles | 90.00, 116.72, 90.00 |
Refinement procedure
| Resolution | 75.180 - 1.700 |
| R-factor | 0.1843 |
| Rwork | 0.183 |
| R-free | 0.21176 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.622 |
| Data reduction software | DIALS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.180 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmeas | 0.083 | 0.953 |
| Number of reflections | 90363 | 4348 |
| <I/σ(I)> | 15 | 1.1 |
| Completeness [%] | 99.8 | 95.9 |
| Redundancy | 6.9 | 5.6 |
| CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 38% v/v 1,4-Dioxane |
