8J1M

Crystal structure of a crosslinked variant of BbZIP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	193
Detector technology	PIXEL
Collection date	2018-02-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	116.407, 51.012, 51.817
Unit cell angles	90.00, 113.92, 90.00

Refinement procedure

Resolution	30.441 - 1.952
R-factor	0.1742
Rwork	0.171
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.972
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.020
High resolution limit [Å]	1.950	1.950
Rmeas	0.179
Number of reflections	36630	1471
<I/σ(I)>	14.35
Completeness [%]	96.4
Redundancy	5.5
CC(1/2)		0.566

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		294	100 mM NaCl, 100 mM CaCl2, pH 7.0 100 mM HEPES, 30% PEG 400