8J0K
Crystal structure of human TFAP2A in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 124.261, 124.261, 96.926 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.900 - 2.100 |
| R-factor | 0.16634 |
| Rwork | 0.165 |
| R-free | 0.19555 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.808 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.040 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.096 | |
| Number of reflections | 50700 | 4095 |
| <I/σ(I)> | 23.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 20.2 | |
| CC(1/2) | 0.999 | 0.952 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 34% v/v PEG 200 , 0.1 M Citric acid pH 3.5 |
