8IYI

Spermidine synthase from Kluyveromyces lactis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	65.252, 98.180, 102.134
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.980 - 1.900
R-factor	0.1692
Rwork	0.168
R-free	0.19870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.818
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	52396	5161
<I/σ(I)>	33
Completeness [%]	100.0
Redundancy	12.9
CC(1/2)	0.996	0.965

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	9.2% v/v TacsimateTM pH 5.0, 16.5% w/v PEG 3350