8IXW

Tilapia lake virus S8 segment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	298.15
Detector technology	CCD
Collection date	2020-12-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 1
Unit cell lengths	50.486, 51.342, 52.706
Unit cell angles	106.86, 108.92, 108.02

Refinement procedure

Resolution	20.960 - 1.800
R-factor	0.1776
Rwork	0.176
R-free	0.20320
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.865
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.960	1.864
High resolution limit [Å]	1.800	1.800
Rmerge	0.039	0.211
Rmeas	0.046	0.247
Rpim	0.024	0.127
Number of reflections	38281	3781
<I/σ(I)>	20.12
Completeness [%]	96.2
Redundancy	3.6
CC(1/2)	0.998	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289.15	0.08 M Potassium chloride, 0.02 M Barium chloride dihydrate, 0.04 M Sodium cacodylate trihydrate pH 6.0, 40% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.012 M Spermine tetrahydrochloride.