8IWB
Crystal structure of Q9PR55 at pH 7.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-12-16 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.404, 54.156, 76.664 |
| Unit cell angles | 90.00, 92.28, 90.00 |
Refinement procedure
| Resolution | 28.780 - 2.420 |
| R-factor | 0.2403 |
| Rwork | 0.238 |
| R-free | 0.28220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.915 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.780 | 2.511 |
| High resolution limit [Å] | 2.420 | 2.425 |
| Rmerge | 0.084 | 0.742 |
| Rmeas | 0.090 | 0.796 |
| Rpim | 0.033 | 0.286 |
| Number of reflections | 22464 | 2318 |
| <I/σ(I)> | 19.3 | 2 |
| Completeness [%] | 95.9 | 100 |
| Redundancy | 7.5 | 7.6 |
| CC(1/2) | 0.903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | sodium HEPES, sodium dihydrogen phosphate, sodium dihydrogen phosphate |
