8IVL
FABP7 complexed with Cholesterol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2019-03-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.680, 54.910, 66.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.360 - 2.700 |
| R-factor | 0.2302 |
| Rwork | 0.227 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.033 |
| RMSD bond angle | 1.160 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.360 | 2.800 |
| High resolution limit [Å] | 2.600 | 2.700 |
| Rmerge | 0.043 | 0.043 |
| Number of reflections | 3750 | 357 |
| <I/σ(I)> | 58.48 | |
| Completeness [%] | 99.3 | |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277.15 | 0.1 M Bis-Tris 5.5 25% w/vPEG 3350 |
