8IU6

Crystal structure of peptidyl-tRNA hydrolase mutant from Enterococcus faecium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2023-03-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	C 1 2 1
Unit cell lengths	130.739, 49.435, 65.500
Unit cell angles	90.00, 107.87, 90.00

Refinement procedure

Resolution	32.110 - 2.900
Rwork	0.235
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7y52
RMSD bond length	0.003
RMSD bond angle	0.881
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.340	3.010
High resolution limit [Å]	2.900	2.900
Number of reflections	9021	861
<I/σ(I)>	21.7
Completeness [%]	93.0
Redundancy	2.1
CC(1/2)	0.980	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1M Tris-HCl pH 7.5 0.2M Ammonium Sulfate 25% PEG 4K