8ITP

Crystal structure of USP47 catalytic domain complex with ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	298
Detector technology	CCD
Collection date	2014-10-21
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	46.957, 93.399, 217.762
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.510 - 3.000
R-factor	0.25
Rwork	0.246
R-free	0.28430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.450
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.140	0.066	0.374
Total number of observations	80505
Number of reflections	19209	2093	1776
<I/σ(I)>	6.6
Completeness [%]	96.4	96.1	91.4
Redundancy	4.2	5.3	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	100 mM bis-Tris (pH 5.5), 25% PEG 3350, 50 mM MgCl2