Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9788 |
| Spacegroup name | I 4 |
| Unit cell lengths | 107.620, 107.620, 75.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.315 - 2.100 |
| R-factor | 0.2281 |
| Rwork | 0.226 |
| R-free | 0.26660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.143 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.044 | 0.837 |
| Rmeas | 0.046 | 0.910 |
| Number of reflections | 25053 | 1855 |
| <I/σ(I)> | 32.3 | 3.43 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 13.4 | 14.1 |
| CC(1/2) | 1.000 | 0.874 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M Bis-Tris,1M sodium citrate |
