8IQV

Asterias forbesii ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.2915
Spacegroup name	I 4 2 2
Unit cell lengths	116.520, 116.520, 205.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.610 - 1.910
R-factor	0.2203
Rwork	0.219
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.827
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.610	2.010
High resolution limit [Å]	1.910	1.910
Number of reflections	103947	103947
<I/σ(I)>	6.9
Completeness [%]	99.4
Redundancy	10
CC(1/2)	0.997	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM imidazole (pH 8.0), 15%(v/v) reagent alcohol, 200 mM magnesium chloride