8IQM

Structural basis of the specificity and interaction mechanism of Bmf binding to pro-survival proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-19
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	45.465, 56.294, 62.673
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.337 - 1.967
R-factor	0.1999
Rwork	0.196
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.521
Data reduction software	autoPROC
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.9-1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.340	2.037
High resolution limit [Å]	1.967	1.967
Number of reflections	21592	1172
<I/σ(I)>	2.77
Completeness [%]	98.0
Redundancy	8.5
CC(1/2)	0.996	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M potassium thiocyanate, 20% polyethylene glycol monomethyl ether 2,000.