8IQK
Structural basis of the specificity and interaction mechanism of Bmf binding to pro-survival proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-09 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 102.524, 61.668, 102.528 |
| Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
| Resolution | 39.425 - 2.879 |
| R-factor | 0.2285 |
| Rwork | 0.226 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.679 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.425 | 2.982 |
| High resolution limit [Å] | 2.879 | 2.879 |
| Number of reflections | 27310 | 2523 |
| <I/σ(I)> | 8.92 | |
| Completeness [%] | 93.0 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.991 | 0.655 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M sodium chloride, 0.1 M sodium acetate: acetic acid, pH 4.5, 1.26 M ammonium sulfate |
