8IQB

Crystal structure of AsfvPrimPol N-terminal Prim/Pol domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-20
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	43.829, 73.610, 78.927
Unit cell angles	90.00, 95.39, 90.00

Refinement procedure

Resolution	20.710 - 2.580
R-factor	0.2205
Rwork	0.217
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.003
RMSD bond angle	0.647
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.720	2.720
High resolution limit [Å]	2.580	2.580
Rmerge	0.129	0.602
Rmeas	0.158	0.741
Rpim	0.089	0.427
Total number of observations		6753
Number of reflections	28321	2295
<I/σ(I)>	5.2	2.4
Completeness [%]	98.5
Redundancy	3	2.9
CC(1/2)	0.981	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Bis-Tris propane pH 8.5, 20% PEG 3350 and 0.2 M Sodium iodide