8IPM

The structure of human mitochondrial methyltransferase METTL15 with h44_RNA, RBFA and SAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	71.220, 134.111, 93.026
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.528 - 3.100
R-factor	0.2564
Rwork	0.253
R-free	0.28780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.537
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.530	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.105	0.616
Number of reflections	8176	888
<I/σ(I)>	14.7	3.7
Completeness [%]	97.4
Redundancy	9
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	20% PEG 4000, 0.2 M ADA (pH 6.4)