8IP1

Escherichia coli OpgD mutant-D388N with beta-1,2-glucan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	58.092, 87.099, 110.853
Unit cell angles	90.00, 101.13, 90.00

Refinement procedure

Resolution	47.740 - 2.060
Rwork	0.175
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.597
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.740	2.110
High resolution limit [Å]	2.060	2.060
Number of reflections	66585	4130
<I/σ(I)>	12.8
Completeness [%]	99.3
Redundancy	6.5
CC(1/2)	0.996	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MMT (pH 4.0), PEG1500, Beta-1,2-glucan