8IOX

Escherichia coli OpgD mutant-D388N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	C 2 2 2
Unit cell lengths	226.750, 392.760, 324.648
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.143 - 2.950
Rwork	0.194
R-free	0.23220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.602
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.143	3.000
High resolution limit [Å]	2.950	2.950
Rmerge	0.208
Number of reflections	301232	14824
<I/σ(I)>	13
Completeness [%]	100.0
Redundancy	13.7
CC(1/2)	0.994	0.869

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG2000MME, TMAO, tris-HCl (pH 8.5)