8IMS
Crystal structure of TRAF7 coiled-coil domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978530 |
| Spacegroup name | P 64 |
| Unit cell lengths | 169.923, 169.923, 57.055 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.090 - 3.300 |
| R-factor | 0.2624 |
| Rwork | 0.262 |
| R-free | 0.27320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.661 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.090 | 3.500 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 14379 | 1393 |
| <I/σ(I)> | 8.22 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.07 | |
| CC(1/2) | 0.996 | 0.527 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1M HEPES pH6.9, 0.2M magnesium formate dihydrate |
