8IM8

Crystal structure of Periplasmic alpha-amylase (MalS) from E.coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-30
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	H 3
Unit cell lengths	133.242, 133.242, 386.269
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.020 - 2.700
R-factor	0.1796
Rwork	0.178
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ea9 5a2a
RMSD bond length	0.010
RMSD bond angle	1.211
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.020	2.770
High resolution limit [Å]	2.700	2.700
Rmeas	0.123	0.684
Number of reflections	69932	4802
<I/σ(I)>	168	5.5
Completeness [%]	99.8	98.5
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1M Sodium citrate tribasic dihydrate pH5.5 20% w/v Polyehtylene glycol 1000 0.1M Lthium sulfate monohydrate