8ILF

The crystal structure of dGTPalphaSe-Sp:DNApre-II:Pol X substrate ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-04
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.977
Spacegroup name	P 21 21 21
Unit cell lengths	59.666, 78.190, 101.304
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.20647
Rwork	0.204
R-free	0.24462
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.385
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.110
High resolution limit [Å]	2.300	6.440	3.000
Rmerge	0.117	0.098	1.225
Number of reflections	21788	1180	2123
<I/σ(I)>	7.8
Completeness [%]	99.9	99.7	100
Redundancy	12.7	11.6	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	ammonium sulfate (0.2 M), Bis-Tris pH 5.5 (0.1 M), 20% PEG8000