8IL7

Structure of human soluble Adenylyl Cyclase in complex with lactate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2022-10-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 63
Unit cell lengths	99.280, 99.280, 98.564
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.640 - 1.950
R-factor	0.1642
Rwork	0.163
R-free	0.18850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.322
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.640	2.050
High resolution limit [Å]	1.950	1.950
Number of reflections	40207	5705
<I/σ(I)>	20.3
Completeness [%]	99.9
Redundancy	14.3
CC(1/2)	0.998	0.857

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.8	289.15	0.2 M Sodium citrate tribasic, 15% PEG 3350, 0.1 M sodium lactate pH 3.8, 10% glycerol