8IL0
Crystal structure of LmbT from Streptomyces lincolnensis NRRL ISP-5355
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979021 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 103.741, 103.741, 197.251 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.310 - 2.810 |
| R-factor | 0.2328 |
| Rwork | 0.231 |
| R-free | 0.25730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.242 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.820 | 2.960 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Number of reflections | 12924 | 1925 |
| <I/σ(I)> | 23 | |
| Completeness [%] | 95.3 | |
| Redundancy | 10 | |
| CC(1/2) | 0.999 | 0.831 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Magnesium acetate tetrahydrate, 0.1 M MES, pH6.5 10 %, w/v PEG 10000 |
