8IL0

Crystal structure of LmbT from Streptomyces lincolnensis NRRL ISP-5355

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979021
Spacegroup name	I 41 2 2
Unit cell lengths	103.741, 103.741, 197.251
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.310 - 2.810
R-factor	0.2328
Rwork	0.231
R-free	0.25730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.242
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.820	2.960
High resolution limit [Å]	2.810	2.810
Number of reflections	12924	1925
<I/σ(I)>	23
Completeness [%]	95.3
Redundancy	10
CC(1/2)	0.999	0.831

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Magnesium acetate tetrahydrate, 0.1 M MES, pH6.5 10 %, w/v PEG 10000