8IKE

Transcription factors LMX1a mutant-R199A homeobox domain complex with Wnt1 promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	46.637, 47.303, 95.912
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.300 - 2.600
R-factor	0.2313
Rwork	0.228
R-free	0.26240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.646
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (3.3.22)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.300	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.091	0.603
Number of reflections	6915	664
<I/σ(I)>	19.8	4.6
Completeness [%]	99.6	99.7
Redundancy	12.3	12
CC(1/2)	0.997	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	20% PEG 4000, 0.2 mM MES monohydrate (PH 5.9)