8IK6

pUbl depleted Parkin complex with pUbiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.8856
Spacegroup name	P 64 2 2
Unit cell lengths	187.805, 187.805, 141.857
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.150 - 3.300
Rwork	0.234
R-free	0.26985
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n2w
RMSD bond length	0.005
RMSD bond angle	1.061
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.150	3.560
High resolution limit [Å]	3.300	3.300
Rmerge	0.193
Number of reflections	20948	4366
<I/σ(I)>	8.4
Completeness [%]	92.8
Redundancy	7.1
CC(1/2)	0.997	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	1.6 M Ammonium sulfate, 0.1 M MES monohydrate pH 6.5, 10% v/v 1,4-Dioxane