8IK5

Transcription factor LMX1a homeobox domain in complex with Wnt1 promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	46.290, 46.419, 96.098
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.077 - 1.989
R-factor	0.2081
Rwork	0.207
R-free	0.23610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.952
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (3.3.22)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.080	2.030
High resolution limit [Å]	1.989	2.000
Rmerge	0.065	0.607
Number of reflections	14659	707
<I/σ(I)>	33.7	2.3
Completeness [%]	99.1
Redundancy	10.3
CC(1/2)	0.994	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	27% PEG MME 2000, 0.1 mM sodium cacodylate (pH 6.5)