8IJU

ATP-dependent RNA helicase DDX39A (URH49delta41)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-22
Detector	RAYONIX MX-225
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	34.974, 96.606, 59.755
Unit cell angles	90.00, 95.51, 90.00

Refinement procedure

Resolution	37.500 - 1.820
R-factor	0.1862
Rwork	0.184
R-free	0.22190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.859
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.820	1.820
Rmerge	0.046	0.373
Rmeas	0.058	0.481
Number of reflections	68537	10621
<I/σ(I)>	12.4	2.09
Completeness [%]	98.5	94.2
Redundancy	2.4	2.1
CC(1/2)	0.998	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2 mM Na2PO4, 30 % (w/v) PEG3350