8IJT
crystal structure of Hyp N135A mutant from Hypoxylon sp. E7406B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-02 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.872, 86.230, 102.349 |
| Unit cell angles | 77.50, 85.91, 87.69 |
Refinement procedure
| Resolution | 31.370 - 2.560 |
| R-factor | 0.2031 |
| Rwork | 0.201 |
| R-free | 0.25170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.778 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.437 | 2.602 |
| High resolution limit [Å] | 2.558 | 2.558 |
| Number of reflections | 48170 | 2372 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 97.7 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.998 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 13% PEG4000, 0.1 M Na3Cit, pH5.6, 0.15 M NaCl, 0.01 M MgCl2 |
