8IIU
anti-VEGF nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.468, 39.369, 39.952 |
| Unit cell angles | 102.14, 90.23, 90.95 |
Refinement procedure
| Resolution | 24.590 - 1.270 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.18690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5imk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.910 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.590 | 1.315 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Number of reflections | 46593 | 4515 |
| <I/σ(I)> | 16 | |
| Completeness [%] | 94.0 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.974 | 0.743 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294.15 | (NH4)2SO4, HEPES (pH=7.5), NDSB-195 |
