8IIG
Complex form of MsmUdgX H109A mutant and uracil- obtained from uracil DNA (ttUtt) post its cleavage by UdgX H109A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-09-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.710, 50.720, 55.060 |
| Unit cell angles | 90.00, 104.50, 90.00 |
Refinement procedure
| Resolution | 26.650 - 2.300 |
| R-factor | 0.1877 |
| Rwork | 0.186 |
| R-free | 0.22220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.610 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.110 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.140 | 0.430 |
| Rmeas | 0.190 | 0.590 |
| Rpim | 0.130 | 0.390 |
| Number of reflections | 8832 | 848 |
| <I/σ(I)> | 5.6 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.7 |
| CC(1/2) | 0.982 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 295 | 2.0M Ammonium citrate tribasic pH7.0, 0.1M BIS-TRIS propane pH7.0 |
