8IIB

Crystal structure of Israeli acute paralysis virus RNA-dependent RNA polymerase delta85 mutant (residues 86-546)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 31 2 1
Unit cell lengths	150.510, 150.510, 104.626
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.410 - 2.690
R-factor	0.2147
Rwork	0.213
R-free	0.25390
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	23.800	23.800	2.800
High resolution limit [Å]	2.690	5.790	2.690
Rmerge	0.041	0.020	0.713
Rmeas	0.043	0.021	0.749
Rpim	0.014	0.007	0.229
Number of reflections	38083	3954	3746
<I/σ(I)>	16.2
Completeness [%]	100.0	99.6	100
Redundancy	10.1	9.6	10.6
CC(1/2)		0.999	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	289	PEG 4000