8IHW
X-ray crystal structure of D43R mutant of endo-1,4-beta glucanase from Eisenia fetida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.292, 71.682, 55.359 |
| Unit cell angles | 90.00, 114.23, 90.00 |
Refinement procedure
| Resolution | 48.600 - 1.700 |
| R-factor | 0.1455 |
| Rwork | 0.144 |
| R-free | 0.17580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.754 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmeas | 0.134 | 0.499 |
| Number of reflections | 41828 | 2194 |
| <I/σ(I)> | 11.6 | 3.6 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 6.8 | |
| CC(1/2) | 0.996 | 0.921 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 16% PEG3350, 0.2 M magnesium chloride, 50 mM sodium acetate pH 5.0 |
