8IH8
anti-sigmaF factor and Anti-sigmaF factor antagonist complex(usfx-RsfB)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-12-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.425, 77.928, 133.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.650 - 2.000 |
R-factor | 0.1824 |
Rwork | 0.180 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.390 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.060 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.057 | |
Number of reflections | 36351 | 21537 |
<I/σ(I)> | 42.7 | |
Completeness [%] | 99.3 | |
Redundancy | 12.7 | |
CC(1/2) | 0.150 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.05M HEPES pH7.5 0.2M KCl 35.5% v/v pentaerythritol propoxylate (5/4 PO/OH) |