8IH7
AmnG-AmnH complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.029, 252.288, 60.785 |
| Unit cell angles | 90.00, 114.94, 90.00 |
Refinement procedure
| Resolution | 20.110 - 2.480 |
| R-factor | 0.1749 |
| Rwork | 0.173 |
| R-free | 0.22270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.937 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.070 | 2.550 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Number of reflections | 52921 | 52921 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.996 | 0.911 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289 | 0.2M magnesium chloride hexahydrate,0.2 M Tris 8.5,20% PEG 3350 |
