8IE2

Crystal structure of Lactiplantibacillus plantarum GlyRS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	155.140, 163.170, 155.280
Unit cell angles	90.00, 119.81, 90.00

Refinement procedure

Resolution	48.630 - 3.600
R-factor	0.2217
Rwork	0.215
R-free	0.24420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.852
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.690
High resolution limit [Å]	3.600	16.100	3.600
Rmerge	0.258	0.062	0.678
Rmeas	0.279	0.068	0.735
Number of reflections	46387	819	691
<I/σ(I)>	6.82
Completeness [%]	59.5
Redundancy	6.8
CC(1/2)	0.989	0.997	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	12% PEG3350, 4% Tacsimate pH 5.0