8IC9
Lys48-linked K48C-diubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2021-07-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 86.790, 31.191, 25.272 |
| Unit cell angles | 90.00, 106.93, 88.65 |
Refinement procedure
| Resolution | 19.641 - 1.250 |
| Rwork | 0.186 |
| R-free | 0.21880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.574 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.641 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.062 | 0.554 |
| Number of reflections | 66682 | 11849 |
| <I/σ(I)> | 6.7 | 2.7 |
| Completeness [%] | 95.1 | 93.6 |
| Redundancy | 3.6 | |
| CC(1/2) | 0.992 | 0.911 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | 38% 2-methyl-2,4-pentanediol and 50 mM sodium citrate (pH 4.0) |
