8IC7

exo-beta-D-arabinofuranosidase ExoMA2 from Microbacterium arabinogalactanolyticum in complex with beta-D-arabinofuranose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-28
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	66.763, 97.249, 139.681
Unit cell angles	90.00, 101.25, 90.00

Refinement procedure

Resolution	45.870 - 1.350
R-factor	0.1601
Rwork	0.159
R-free	0.18244
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.651
Data reduction software	XDS (Jan 10, 2022)
Data scaling software	Aimless (0.7.9)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.620	1.370
High resolution limit [Å]	1.350	1.350
Rmerge	0.056	0.965
Rpim	0.036	0.629
Number of reflections	382283	18879
<I/σ(I)>	9.5	1
Completeness [%]	99.9
Redundancy	3.4
CC(1/2)	0.988	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.9	293.15	0.2 M MgCl2, 0.1 M CHES-NaOH (pH 8.9), 14% PEG8000, 100 mM D-arabinose