8IBS

Crystal structure of GH42 beta-galactosidase BiBga42A from Bifidobacterium longum subspecies infantis E160A/E318A mutant in complex with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	234.470, 182.691, 143.263
Unit cell angles	90.00, 125.73, 90.00

Refinement procedure

Resolution	48.610 - 1.900
R-factor	0.18935
Rwork	0.187
R-free	0.22841
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.405
Data reduction software	XDS (Jan 10, 2022)
Data scaling software	Aimless (0.7.9)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.570	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.097	0.935
Rpim	0.058	0.672
Number of reflections	376638	15738
<I/σ(I)>	9.5	1.1
Completeness [%]	98.3	83
Redundancy	3.7	2.8
CC(1/2)	0.997	0.545

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	0.1 M MES-NaOH buffer (pH 6.5), 0.1 M (CH3COO)2Mg, 10% PEG 10000, 25% glycerol, 30 mM lacto-N-tetraose (cleaved)