8IBS
Crystal structure of GH42 beta-galactosidase BiBga42A from Bifidobacterium longum subspecies infantis E160A/E318A mutant in complex with galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 234.470, 182.691, 143.263 |
Unit cell angles | 90.00, 125.73, 90.00 |
Refinement procedure
Resolution | 48.610 - 1.900 |
R-factor | 0.18935 |
Rwork | 0.187 |
R-free | 0.22841 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.405 |
Data reduction software | XDS (Jan 10, 2022) |
Data scaling software | Aimless (0.7.9) |
Phasing software | MOLREP (11.9.02) |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.570 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.097 | 0.935 |
Rpim | 0.058 | 0.672 |
Number of reflections | 376638 | 15738 |
<I/σ(I)> | 9.5 | 1.1 |
Completeness [%] | 98.3 | 83 |
Redundancy | 3.7 | 2.8 |
CC(1/2) | 0.997 | 0.545 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.1 M MES-NaOH buffer (pH 6.5), 0.1 M (CH3COO)2Mg, 10% PEG 10000, 25% glycerol, 30 mM lacto-N-tetraose (cleaved) |