8I7H
Meso-Diaminopimelate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-20 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.541 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 213.300, 213.300, 245.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.000 - 3.020 |
| R-factor | 0.20905 |
| Rwork | 0.207 |
| R-free | 0.24335 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.440 |
| Refinement software | PHENIX (V1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.290 | 3.140 |
| High resolution limit [Å] | 3.020 | 3.020 |
| Number of reflections | 40836 | 4541 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 97.0 | 96.4 |
| Redundancy | 5.7 | 4.4 |
| CC(1/2) | 0.980 | 0.701 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293.15 | 0.1M Sodium acetate trihydrate,2M ammonium sulfate, pH 4.5 |
