8I6K
Structure of hMNDA HIN with dsDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-24 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 |
| Unit cell lengths | 40.652, 40.652, 138.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 13.070 - 2.400 |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.25310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.330 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.15_3459: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.070 | 2.486 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.038 | 0.356 |
| Rmeas | 0.042 | 0.422 |
| Rpim | 0.019 | 0.223 |
| Number of reflections | 9936 | 955 |
| <I/σ(I)> | 30.5 | 2.94 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 4.8 | 3.4 |
| CC(1/2) | 0.999 | 0.864 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.1 M Sodium malonate pH 8.0, 0.1 M Tris pH 8.0, 30% w/v Polyethylene glycol 1000 |
