8I60

Crystal structure of GAS41 YEATS domain in complex with histone H3K27cr

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	91.980, 91.980, 83.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.790 - 2.300
R-factor	0.2579
Rwork	0.256
R-free	0.29110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.202
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	BALBES
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.790	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	16481	2342
<I/σ(I)>	11.5	5
Completeness [%]	100.0
Redundancy	11.9
CC(1/2)	0.995	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288	Ammonium sulfate